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Fig. 5

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Publication: Manzoli et al., 2024 - Mucopolysaccharidosis type II zebrafish model exhibits early impaired proteasomal-mediated degradation of the axon guidance receptor Dcc
All Figures: Figures for Manzoli et al., 2024

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Fig. 5 Proteasome inhibition in wild-type primary neurons results in Dcc upregulation.

A Western blot analysis on 2 DIV wild-type and ids mutant fish-derived primary neurons showed significant upregulation of polyubiquitinated proteins and P62, as depicted by the bar graphs on the right. On the other hand, no differences were found for Lc3-II (n = 6 biological replicates). B Wild-type fish-derived primary neurons were treated with MG-132 (proteasome inhibitor) for 1, 6, or 16 hours and then analysed at 2 DIV. On the left, representative western blots for Dcc, ubiquitin and P62. After 16 hours of treatment, Dcc levels were significantly increased when compared to DMSO (n = 3 independent experiments). Data are expressed as the mean ± SD (*p < 0.05; **p < 0.005; ***p < 0.001; t-test).

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