Fig. 2.

Fig. 2.

Medaka lack an endogenous injury-induced interferon response. (A) Gene expression heatmap showing scaled average gene expression for 12 interferon-stimulated genes in the indicated species and tissue type at each time point. (B) RNA in situ hybridization of isg15 (interferon-responding cells), kdrl (endothelial cells), and myl7 (cardiomyocytes) in ventricle cryosections in the indicated species and time point. Scale bars: 200 µm. Anatomical labels: V, intact ventricle; BA, bulbus arteriosus; W, wound area. Images are representative of at least three individuals at each time point. (C) Gene expression feature plot for ifnphi1 across all zebrafish cardiac cell types, color scale=expression level. (D) Quantification of proportion of zebrafish endothelial cells expressing ifnphi1 at each time point.