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Fig. 5 zeb2a inhibits DC development through repressing id2a expression. (A) Experimental design of zeb2a overexpression. The embryos injected with hsp70:zeb2a constructs and Tol2 transposase (TP) mRNA were heat-shocked at 1 dpf to induce the zeb2a expression. The heat-shocked embryos were collected at 2 dpf for RT-PCR analysis. (B,C) RT-PCR shows the expression levels of zeb2a and id2a in the whole embryo of 2 dpf WT and zeb2a-overexpressed fish. n=4 for each group. (D-F) Representative images (D) and quantification of the ccl34b.1+mpeg1.1+(GFP+DsRedx+) (F) and ccl34b.1−mpeg1.1+ (GFP−DsRedx+) cells (E) and their density in the transverse midbrain sections of 1-month-old TgBAC(ccl34b.1:eGFP);Tg(mpeg1.1:DsRedx) WT or mpeg1.1:zeb2a-injected fish. n=4 and 5 for WT and mpeg1.1:zeb2a-injected fish, respectively. Data are mean±s.d. Unpaired Student's t-test with Welch's correction