IMAGE

Figure 2

ID

ZDB-IMAGE-240405-84

Genes

smpx

Antibodies

Ab1-smpx

Source

Figures for Diana et al., 2024

Image

Figure Caption

Figure 2

Smpx protein localization in the neuromast mechanosensory hair cells. Confocal Z-stacks of immunofluorescence on whole mount 120 hpf larvae. (A–C) Smpx (green) localizes in the most internal cells of the neuromast, namely the mechanosensory hair cells, while it is excluded from the outermost cells (red) of the transgenic line tg (sod1:sod1; hsp70:DsRed) and the presumptive accessory cell layers (white bracket) encompassed in between the outmost DsRed-positive cells and the inmost hair cells. (D–F) Smpx (red) co-localizes with acetylated tubulin (green) in the kinocilium, with Smpx showing a ‘beads-on-a-string’ distribution (arrowheads in D). In some cases, the kinocilium is not labeled by the anti-Smpx antibody (arrow in F). (A–F) Larvae were counterstained with DAPI (blue) to visualize cell nuclei. Lateral views, anterior to the left. Scale bars are 15 µm in A–C and 10 µm in D–F.

Figure Data

Expression / Labeling details

Acknowledgments

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