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Fig. 2 Gli3–CNEs conserved in slowly evolving fish genomes exhibit enhancer activity in Gli3-related tissues. (a) CNEs with highest sequence identity between human and elephant shark drive reproducible GFP transgenic reporter activity in multiple zebrafish embryonic tissues, including the pectoral fin (pf), forebrain (fb), and heart (h). (b) The mm1179 mouse embryonic Gli3 limb enhancer was validated in zebrafish for comparison and drives restricted activity in the pf. (c) CNE17 promotes transcriptional activity exclusively in the fb. Representative images (merged bright field and fluorescent signals) of live zebrafish transgenic embryos are shown and white arrowheads indicate reproducible GFP reporter activities (see also Figure S2 and Table S3). Inlets for elements active in the pf show GFP-only signals (in grayscale). Orientation of embryos is anterior to the left and dorsal to the top, with a lateral view.