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Fig. 4 Generation of ints14 knockout zebrafish model using CRISPR/Cas9 gene editing technology. (A) F0 individuals, upon reaching adulthood, transmitted the ints14 knockout mutation to their offspring, illustrating a high efficiency of targeted mutagenesis for the established lines #2 and #4 by T7E1 assay. (B, C) Results of sequencing found that F1_#2 and F1_#4 individuals exhibited 9 and 6 bp deletions, respectively; thus, frameshift mutation in ints14 led to a modification of the termination site, resulting in the early termination of the ints14 protein.
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