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Figure Caption
Fig. 3 Test of activity for ints14 RGEN in zebrafish. (A) To identify the efficiency of the sgRNA, we co-injected ints14 sgRNA (RG1) and Cas9 protein. Co-injected embryos showed that the sgRNA efficiently generated mismatched DNA when the T7 endonuclease I (T7E1) assay was performed. (B) The TA-cloning and sequencing results demonstrate that a deletion of approximately 12 bp can be achieved via the RGEN system.
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