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Fig. 1 Stage-dependent expression of zebrafish ints14 mRNA. (A) RT-PCR analysis of ints14 expression in developing zebrafish embryo. The number indicates different development stages as hour post fertilization (hpf). Embryos of indicated stages were collected and applied to RT-PCR using beta-actin as internal control. (B) Whole-mount in situ hybridization analysis of ints14 expression in zebrafish embryo at the indicated developmental stage. All panels are dorsal, top, or lateral views with animal poles up or anterior to the left. Scale bars indicate 250μm. RT-PCR, reverse transcription polymerase chain reaction.