IMAGE

Fig. 1

ID: ZDB-IMAGE-240404-26
Publication: Hossain et al., 2024 - Establishment of a graphene quantum dot (GQD) based steroid binding assay for the nuclear progesterone receptor (pgr)
All Figures: Figures for Hossain et al., 2024

Image

Figure Caption

Fig. 1

Expression and purification of the ligand-binding domain (LBD) of zebrafish Pgr as a fusion protein with GST and production of GQD-coupled GST-zPgrLBD.

A. A diagram of the produced recombinant GST-fusion zPgrLBD. The predicted ligand-binding domain at the C-terminus is indicated by black boxes. The GST tag at the N-terminus is indicated by a white box. B. SDS-PAGE analysis of purified recombinant GST-zPgrLBD. Protein bands were detected by CBBR staining (CBBR) or immunostained with anti-GST-tag antibody (α-GST). C. Western blot analysis of GST-zPgrLBD and GQD-GST-zPgrLBD. The protein band of GST-zPgrLBD is indicated by an arrowhead. The bands of GQD-GST-zPgrLBD are indicated by a parenthesis.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem Biophys Rep