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Fig. 4 Zebrafish embryo model of LPS-induced inflammation. (A) Representative images of posterior portions of zebrafish embryos with Sudan Black B staining at 3 days post-fertilization (dpf). The upper panel indicates the control group, the middle panel indicates the LPS-induced inflammation group (LPS, 10 μg/mL), and the bottom panel indicates the LPS-induced inflammation group treated with dexamethasone (4 μg/mL). The red dotted area indicates the caudal hematopoietic tissue (CHT). (B) Quantification of the number of Sudan Black B-positive neutrophils in the CHT described in (A). The graph represents the means ± SEM. of individual values. P-values were calculated using an unpaired two-tailed Student's t-test. **P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)