Fig. 2

Fig. 2

Csrp3 is involved in trabeculation, and its expression is regulated by hemodynamics and Notch signaling during heart development. A Upper: visualization of trabeculae using Tg(vmhc:mCherry-NTR) displayed increased trabeculation in 218A larval hearts. Scale bars, 20 μm. Bottom: Quantification of the number or density of trabeculae in wild-type and 218A larval hearts at indicated stages. N = 9 each. Data are presented as mean ± SD, Student’s t-test, **, p < 0.01. B Upper: HE staining showed an increased myocardial density in 218A adult hearts. Scale bars, 100 μm. Bottom: Quantification of the myocardial density in wild-type and 218A adult hearts. N = 6 each. Data are presented as mean ± SD, Student’s t-test, ***, p < 0.001. C Comparison of the fluorescence pattern in the hearts of + /218A and Tg(tp1:d2GFP) larvae. Scale bars, 20 μm. D, E WISH and confocal images showed that inhibition of Notch signaling with DATP treatment enhanced both endogenous csrp3 expression in wild-type hearts (D) and GFP fluorescence in + /218A hearts (E). Scale bars, 20 μm. F Confocal and WISH images showed that reducing blood flow via tnnt2a MO injection markedly suppresses both GFP fluorescence in + /218A hearts and endogenous csrp3 expression in wild-type hearts. Scale bars, 20 μm