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Fig. 2 Metabolic rates of parents (F0) and offspring (F1). Parent metabolic rates (A, B, E, F) were influenced by genotype (A, B DNMT3a+/+; E, F DNMT3a−/−) on 49, 77, and 105 days post fertilisation (dpf), but not at 189 dpf. Genotype interacted with BPA exposure (control = solid line; BPA exposure = broken line), and metabolic rates changed with temperature (A, E blue symbols = 24 °C; B, F red symbols = 30 °C). In particular, the BPA-induced increase in metabolic rate at 30 °C was not apparent in the DNMT3a−/− genotype. Offspring (F1) metabolic rates were influenced by a genotype (C, D DNMT3a+/+; G, H DNMT3a−/−) × temperature (C, G blue symbols = 24 °C; D, H red symbols = 30 °C) interaction at 49 days post-fertilisation (dpf), and by temperature at 77 dpf. Genotype had a main effect at 105 dpf. Parental BPA exposure = broken line, parental control = solid line. Means ±95 % CI are shown (24 °C: turquoise shading = 95 % CI for BPA treatment, light blue = control; 30 °C: pink shading = 95 % CI BPA treatment, orange = control), and n = 10–12 fish per treatment.