ZFIN Image: Utsumi et al., 2024, FIGURE 6

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FIGURE 6

Decondensation of chromosomes and re-expression of foxa2 in detached cells in mastl^kt441b homozygous embryos. Classification of cells detached from the CNH by foxa2 expression and chromosome condensation in mastl^kt441b homozygous embryos carrying Bac(foxa2/Kaede) transgene at 8 ss, 12 ss and 16 ss. In situ hybridization of foxa2 mRNA and immunostaining with anti-Kaede antibody was carried out. Kaede-positive cells, i.e., cells detached from the CNH, in 4 mastl^kt441b homozygous embryos are classified according to foxa2 mRNA expression and chromosome condensation. Note that most foxa2-expressing cells had decondensed chromosomes, suggesting that foxa2 expression was restarted after completion of mitosis.

Acknowledgments

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