FIGURE 9

FIGURE 9

Transcriptome analysis of bigheart mutant. (A) MA-plot for differential expression analysis in RNA-seq of bh^+/− and bh^−/−, x axis indicates the normalized mean expression (Log2 mean expression) and the y axis indicates the log 2 fold change (Red dots: upregulated genes and the blue dots: downregulated genes) (Log₂ normal count). (B) Heat map showing differential expression of selected cardiac genes in bh^+/− and bh^−/− heart tissue (Log₂ normal count). (C) Western blot analysis of calcium handling gene, Camk2d1 and cardiac remodeling gene, Hdac1 in adult heart tissue. (D) Quantification of Western blot in (C). (E) mRNA expression of calcium handling genes ryr2b and atp2a2a in bh^+/− and bh^−/− heart. RT PCR analyses were performed three times in triplicates. **p < 0.01 and ***p < 0.001. The bars indicate the average values ± SD.