Sex ratios and gonad histology in adult ZW and ZZ zebrafish at 5 months post fertilization (mpf). (A) Sex phenotypes in progeny of ZW females crossed to ZZ males. Within each sex genotype, each point represents a different cross. No ZZ individuals became adult females. (B) Sex phenotypes in progeny of ZW females crossed to ZW neomales. No ZZ individuals became adult females. C-G. Cross sections of 5mpf adult gonads. (C) ZZ adult testis. (D) ZW ovary. (E) ZW neomale testis. (F) WW ovary. (G) WW neomale testis. Ovary histology for ZW and WW fish showed no clear difference and testis histology of ZZ, ZW, and WW fish was the same. Abbreviations: o, oocyte; pc, pycnotic cell; sc, spermatocyte; sg, spermatogonium; sp, spermatid; sz, spermatozoa.
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