|
Fig. 4 Proliferation does not contribute to the regeneration of supernumerary hair cells in krm1nl10 neuromasts (A) Timeline of neomycin expose at 5dpf, followed by 24 h of BrdU incubation at 5-6dpf, 6-7dpf, or 7-8dpf, all conditions were allowed to develop until 8dpf then fixed and processed for imaging. (B–G‴’’) Confocal projections of L2 neuromasts at 8dpf expressing Tg(myosin6b:GFP)w186 to label regenerated hair cells (myo6:GFP; green), BrdU incorporation (red), and DAPI labeling of nuclei (blue) at 5-6dpf in heterozygous sibling or krm1nl10 mutant neuromasts NMs, from 6-7dpf,or from 7-8dpf. Scale bar = 20 μm. (H–J) Total numbers of BrdU-positive cells in heterozygous or krm1nl10 mutant neuromasts, percentage of total BrdU-positive cells (total BrdU+ cells/total DAPI+ cells), and percentage of BrdU-positive hair cells (BrdU+ myo6:GFP+ cells/total myo6:GFP+ cells). 5-6dpf: het sibling n = 50 NMs (12 fish), krm1nl10 n = 46 NM (11 fish); 6-7dpf: het sibling n = 34 NMs (9 fish), krm1nl10 n = 39 NM (9 fish); 7-8dpf: het sibling n = 50 NMs (12 fish), krm1nl10 n = 40 NM (10 fish). All data presented as mean ± SD, Kruskal-Wallis test, Dunn’s multiple comparisons test.