Fig. 1

Fig. 1 krm1 expression is dynamic during posterior lateral line regeneration (A–D) RNA in situ hybridization of krm1 showing expression in 5dpf NM prior to exposure to NEO, 4 h after NEO exposure, 1 day post NEO exposure in a 6dpf larva, and 3 -days post NEO exposure in 8dpf larva. (E–H) Confocal projections of HCR-FISH showing krm1 expression (red) during regeneration in wild-type NMs in Tg(myosin6b:GFP)w186 larvae (green) with nuclei labeled with DAPI (blue) in 5dpf NM prior to exposure to NEO, 4 h after NEO exposure, 1 day post NEO exposure in a 6dpf larva, and 3 days post NEO exposure in a 8dpf larva. (I) Quantification of fluorescence intensity in arbitrary units (A.U.) of HCR krm1 expression normalized to background during regeneration in wild-type larvae. Pre-NEO n = 13 NM (7 fish), 4 h post-NEO n = 11 NM (7 fish), 1 day post-NEO n = 10 NM (7 fish), and 3 days post-NEO n = 14 NM (9 fish). All data presented as mean ± SD, Kruskal-Wallis test, Dunn’s multiple comparisons test. Scale bar = 20 μm.