Figure S3.

Figure S3. Accumulation of hepatic lipid droplets after removal of lipofermata.

(A, B, C)Tg(fabp10a:GFP) zebrafish were treated with 5 μM of lipofermata or 0.01% of DMSO (vehicle) from 4 to 6 dpf fast. The drugs were washed, and the animals were allowed to recover. (A) Maximum-intensity projections of 7 and 8 dpf fasting Tg(fabp10a:GFP) (pink) with Nile Red staining (yellow) treated with 5 μM of lipofermata or 0.01% of DMSO (vehicle) from 4 to 6 dpf fast. Scale bar = 20 μm. (B, C) Barplot with the mean ± SEM of the number of lipid droplets per liver (B) and liver size (C) in vehicle- and lipofermata-treated animals. (D) Surface fish larvae were treated with 2 μM of lipofermata or 0.01% of DMSO (vehicle) from 5 to 7 dpf fast. The drugs were washed, and the animals were allowed to recover for 48 h. Barplot with the mean ± SD of the number of lipid droplets normalized by liver volume in vehicle- and lipofermata-treated surface fish larvae. Each point represents a single animal. *P < 0.05, **P < 0.01, and ***P < 0.001, t test.