Figure 4

Figure 4

The effect of amoxicillin pretreatment on hepatic steatosis induced by short-term fructose diet. (A) Schematic diagram of pretreatment with amoxicillin alone followed by early short-term glucose or fructose diet. (B,C) Quantification of triglyceride and total cholesterol in 7 dpf larvae subject to short-term glucose or fructose diet with amoxicillin pretreatment. 3 independent experiments. Mean + s.e.m. In the ANOVA analysis, ns—not significant. (D) Quantification of hepatic steatosis by ORO staining in 7 dpf larvae. In the Chi-square test, ns—not significant, ** indicates p < 0.01. (E) H&E staining of liver tissues at 7 dpf. Arrows point to fat vacuoles. Scale bar: 5 µm. (F–H) Quantification of the expression of fasn, cidec, and lpin1a at 7 dpf by real-time PCR. Four independent experiments. Mean + s.e.m. In the ANOVA analysis, ns—not significant. dpf—days post-fertilization; AMX—amoxicillin; GLU—glucose; FRU—fructose.