Amoxicillin treatment does not affect zebrafish liver development. (A) Schematic diagram of amoxicillin treatment. (B) Whole-mount in situ hybridization (WISH) revealed the expression of foxa3, gata6 and hhex at 2 dpf after amoxicillin treatment. Ventral view, anterior to the top. L—liver (red dashed lines); P—pancreas (green dashed lines). Numbers indicate the ratio of representative staining observed. (C) Liver (red) size at 5 dpf after amoxicillin treatment as shown in the transgenic reporter line Tg(fabp10a:mCherry; ela:eGFP). Lateral view, anterior to the left. (D) Quantification of relative liver size at 5 dpf. The numbers of larvae used for each condition are indicated. In the ANOVA analysis, ns—not significant. Scale bars, 100 µm. AMX—amoxicillin; dpf—days post-fertilization.
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