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Fig. 4 Single-cell RNA-sequencing and immunostaining. (A) Single-cell RNA-sequencing data from inducible pluripotent stem cell-derived human fetal liver organoids. Spindle plots show expression of AFAP1 (upper panel) and TUSC3 (lower panel) in progenitors of hepatocytes, biliary epithelial cells, stellate cells and endothelial cells (H, BEC, Stel, EC, respectively). Expression of both genes is enriched in BEC and Stel populations. (B) Immunoperoxidase staining shows expression of AFAP1 (upper panels) and TUSC3 (lower panels) in 18-week-old human fetal liver, normal human liver, and liver samples from individuals with biliary atresia and disease controls (primary familial intrahepatic cholestasis type 1, PFIC1). AFAP1 staining is predominantly seen in cholangiocytes. TUSC3 staining is more diffuse, in cholangiocytes, endothelial cells and other cells.
Reprinted from Journal of hepatology, 79(6), Glessner, J.T., Ningappa, M.B., Ngo, K.A., Zahid, M., So, J., Higgs, B.W., Sleiman, P.M.A., Narayanan, T., Ranganathan, S., March, M., Prasadan, K., Vaccaro, C., Reyes-Mugica, M., Velazquez, J., Salgado, C.M., Ebrahimkhani, M.R., Schmitt, L., Rajasundaram, D., Paul, M., Pellegrino, R., Gittes, G.K., Li, D., Wang, X., Billings, J., Squires, R., Ashokkumar, C., Sharif, K., Kelly, D., Dhawan, A., Horslen, S., Lo, C.W., Shin, D., Subramaniam, S., Hakonarson, H., Sindhi, R., Biliary atresia is associated with polygenic susceptibility in ciliogenesis and planar polarity effector genes, 1385-1395, Copyright (2023) with permission from Elsevier. Full text @ J. Hepatol.