ZFIN Image: Lee et al., 2024, Figure 2

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Figure 2

AP does not affect the NF-κB signaling pathway. (a) 293 T cells were transfected with the pGL4.32 luciferase reporter vector and treated with TNF-α (20 ng/mL) for 5 h with or without AP. Luciferase activity was analyzed using the Promega Bright-Glo™ Luciferase Assay System. (b) LPS-primed J774A.1 cells were treated with AP for 2 h and activated for 30 min with nigericin (10 μM). IL-1β in the supernatants (Sup), phospho-NF-κB and NF-κB in the soluble lysates (Lys) were analyzed using a Western blot.

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