ZFIN Image: Iyer et al., 2024, Fig. 3.

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Figure Caption

Fig. 3.

Microglia are unable to effectively clear endogenous debris in clcn7 mutants. (A) TUNEL assay revealing a significantly greater intensity of apoptotic punctae inside the microglia of clcn7 mutants (n=6, mean=73.3, s.d.=39.2, s.e.m.=4.5) than in clcn7 heterozygotes (n=12, mean=35.5, s.d.=22.2, s.e.m.=1.8). (B) Microglia in clcn7 mutants show an accumulation of NBT:DsRed neuronal punctae. (C) The intensity of DsRed punctae inside microglia was significantly greater in clcn7 mutants (n=13, mean=118, s.d.=42.4, s.e.m.=5.7) relative to clcn7 heterozygotes (n=29, mean=43.2, s.d.=33.4, s.e.m.=2.9). (D) High magnification image showing accumulation of neuronal debris inside microglia. (E) Low magnification image showing accumulation of NBT:DsRed punctae in the midbrain, where microglia are enriched. Images are representative of at least three independent experiments. Two-tailed unpaired t-test with Welch's correction was performed to calculate the significance in all graphs; graphs show mean±s.d. Scale bars: 50 µm. A.U., arbitrary units.

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Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.