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Fig. 4 Inhibition of PI3K/AKT/mTOR pathway alleviates blg intrahepatic bile duct anomalies and reduces PDC-E2 content in blg liver. A: Experimental scheme for chemical treatment under Tg(tp1:Tomato) background. B: Confocal 3D images of the intrahepatic bile ducts in the wild-type and blg mutant after LY294002 and Rapamycin treatment at 5 dpf. This treatment could rescue bile duct phenotype of blg mutant. C: Statistical diagram of intrahepatic bile duct diameter in (B) (per group n ? 11). Data are expressed as mean ± SEM, Student's t-test. D: Immunofluorescence staining of PDC-E2 in blg mutant with DMSO, LY294002, and Rapamycin treatment 5 dpf under Tg(tp1:EGFP) background. The treatment could reduce PDC-E2 content in mutant bile ducts. Scale bars, 50 ?m (B and D). n = number of embryos with indicated phenotype/total analyzed in each class.
Reprinted from Journal of genetics and genomics = Yi chuan xue bao, 50(12), Wu, C., Zhang, W., Luo, Y., Cheng, C., Wang, X., Jiang, Y., Li, S., Luo, L., Yang, Y., Zebrafish ppp1r21 mutant as a model for the study of primary biliary cholangitis, 1004-1013, Copyright (2023) with permission from Elsevier. Full text @ J. Genet. Genomics