Figure 10

Figure 10 FB23-2 suppresses demethylation activity of FTO to inhibit diabetes induced endothelial phenotypes in vitro.

(A) m⁶A dot blot assay of global m⁶A abundance in HUVECs treated with or without FB23-2 (2 µM) using 800 or 400 ng total RNAs. MB staining is used as a loading control. n = 3 per group. (B) Immunoblotting of FTO in HUVECs treated with or without FB23-2. GAPDH is used as an internal control. n = 3 per group. (C) EdU assay on HUVECs treated with NG, HG, as well as HG and FB23-2. n = 3 per group. Scale bar: 60 µm. (D) Scratch test on HUVECs receiving indicated treatments. n = 3 per group. Scale bar: 100 µm. (E) Tube formation analyses on HUVECs treated with NG, HG, as well as HG and FB23-2. n = 3 per group. Scale bar: 100 µm. Data information: Data represent different numbers (n) of biological replicates. Data are shown as mean ± SEM. Two-tailed Student’s t test is used in (A, B). One-way ANOVA followed by Bonferroni’s test is used in (C–E). NS: not significant (p > 0.05); *p < 0.05; **p < 0.01; and ***p < 0.001. Source data are available online for this figure.