Fig. 8

Fig. 8 A pan-species neutrophil maturation signature shows the enrichment of mature neutrophils in BM of metastatic neuroblastoma patients.

a Application of the pan-species neutrophil maturation signatures (M1_pan, M2_pan, M3_pan) derived from maturing zebrafish neutrophils (see Methods for details on signature definition) on bulk RNA sequencing data from 38 bone marrow (NB-BM) samples of patients with metastatic (n = 17, infiltrated) and localized (n = 21, control) neuroblastoma. The heatmap displays the ssGSEA⁵⁷ score of the maturation signatures on RNA-seq samples from 38 neuroblastoma patients with (n = 17) and without (n = 21) tumor cell bone marrow infiltration. b, c Assessment of neutrophil maturation based on nuclear morphology in BM cytospin samples from patients with metastatic (n = 12, infiltrated) and localized (n = 9, control) neuroblastoma. Samples were stained with DNA-intercalator Iridium and anti-CD15-Bi209, analyzed with IMC and counted blinded. c Analysis of the frequency of segmented neutrophils in control NB-BM (mean = 3.48%; range = 0.8−10.1%) versus infiltrated (mean = 13.48%; range 2−41.3%, two-tailed unpaired t test, P = 0.025).