Figure 6

Figure 6

(A) Total distance traveled per second by six dpf zebrafish larvae after 2 days of exposure to 0 (control), 1 ppb, and 100 ppb of tacrolimus during the vibrational startle response assay. The data were analyzed using a two-way ANOVA test with Geisser–Greenhouse correction, followed by Dunnett’s multiple comparisons test. (B) Comparison of the average total distance traveled by the tested zebrafish larvae during the tapping stimuli. The data were analyzed using the Kruskal–Wallis test, followed by Dunn’s multiple comparisons test. The statistical comparisons were performed between the control and each treated group. All data are expressed as the median with 95% CI (n = 96; * p < 0.05, *** p < 0.001, **** p < 0.0001).