Figure 4

Figure 4

(A) Oxygen consumption level per minute in four dpf zebrafish larvae after 1 day of exposure to 0 (control), 1 ppb, and 100 ppb of tacrolimus during the respiratory rate assay. The data were analyzed using a two-way ANOVA test with Geisser–Greenhouse correction and expressed as mean. (B) Comparison of total oxygen consumption of the tested zebrafish larvae. The data were analyzed by using one-way ANOVA, followed with Dunnett’s multiple comparisons test, and are presented as mean with SEM. The statistical comparisons were performed between the control and each treated group (n control and 100 ppb = 53, n 1 ppb = 48; ns = not significant).