Figure 3

Figure 3

Comparison of (A) average and (B) maximum blood flow speed in zebrafish larvae after exposure to 0 (control), 1, and 100 ppb of tacrolimus. The data were analyzed by using a one-way ANOVA test, followed by Dunnett’s multiple comparisons test, and are presented as box and whiskers (min to max). The statistical comparisons were performed between the control and each treated group (n control = 30, n 1 ppb = 27, n 100 ppb = 29). The time chronology of the monitoring of the blood flow velocity in the dorsal aorta of a single representative zebrafish larva from (C) 0 (control), (D) 1, and (E) 100 ppb of the tacrolimus-treated groups.