Figure 9

Figure 9

(A) Locomotor activity patterns of zebrafish that were chronically exposed to 0 (control), 1 ppb, and 100 ppb of tacrolimus during 24 h of observation. The data are expressed as mean with SEM. (B,H) Average speed, (C,I) average angular velocity, (D,J) meandering, (E,K) freezing movement time ratio, (F,L) swimming movement time ratio, and (G,M) rapid movement ratio of the tested fish during the day and night cycles, respectively. The data are expressed as the median with interquartile range and were analyzed using the Kruskal–Wallis test, followed by Dunn’s multiple comparisons test. The statistical comparisons were performed between the control and each treated group (n control and 1 ppb = 12, n 100 ppb = 8; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).