Fig. 6.

Fig. 6. AP-1 interacts with Yap1 and Stat3 in Epi/FBs and ECs, respectively.

(A) Schematic of 4-HT–induced Epi/FB-specific expression of tagged AP-1 and Yap1. The hearts were harvested at 0 and 7 dpa for Co-IP, respectively. (B) Co-IP using uninjured ventricles of the triple transgenic line TgBAC(tcf21:Cre-ERT2); Tg(ubb:loxp-dsRed-loxp-Flag-AP-1); Tg(ubb:loxp-dsRed-loxp-HA-Yap1). Yap1 was immunoprecipitated with anti-HA antibody, and AP-1 was detected by anti-FLAG antibody. A control IgG was used as the negative control for immunoprecipitation. (C and D) Reciprocal Co-IP of AP-1 and Yap1 in Epi/FBs at 7 dpa. IgG was used as the negative control. (E) Schematic of 4-HT–induced EC-specific expression of tagged AP-1 and Stat3. (F) Co-IP of AP-1 and Stat3 in ECs under uninjured conditions. (G and H) Reciprocal Co-IP of AP-1 and Stat3 in ECs during heart regeneration. AP-1 was immunoprecipitated/detected by anti-FLAG antibody, and Stat3 was detected/immunoprecipitated with anti-HA antibody. Control IgG was used as the negative control for immunoprecipitation.