FIGURE 3

FIGURE 3

Eddy currents generated outside the body have no effect on osteoblast or osteoclast activity. (A,B) Representative images of fractured scales in osterix:mCherry; trap:GFP double-transgenic zebrafish exposed to 10 or 30 mT ELF-EMFs in 0.5% NaCl at 24 h.p.fr. White dotted lines and solid lines indicate the fracture site and contour of the zebrafish scale, respectively. Lower panels of trap:GFP show a high magnification view of the fracture site (boxed region). Bars, 200 μm (osterix:mCherry and upper panels of trap:GFP); 100 μm (lower panels of trap:GFP). (C,D) Mean fluorescent intensity of osterix:mCherry per scale and percentage of trap:GFP⁺ area (coverage area) per zebrafish scale in fractured scales from zebrafish unexposed or exposed to 10 or 30 mT ELF-EMFs in freshwater (“Fresh”) or 0.5% NaCl (“NaCl”). The data in fresh water are used from Figures 2C,D. A total of 12–18 scales from 3 zebrafish were used in each condition. Asterisks indicate the p-value in one-way ANOVA with Dunnett’s test. Error bars, s.e.m.; **p < 0.01, ***p < 0.001, n.s., not significant.