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Fig. 4 Maintenance of lineage identities during regeneration. (a) Schematic illustration of the experimental procedure. The first fin amputation was performed to inspect the lineages of EGFP+ cells in the respective fin rays. After 2 weeks, the same fin was regenerated to examine the identities of EGFP+ cells in the regenerates. (b) Sections of a fin ray containing EGFP+ OMPs (arrowheads) before and after regeneration. EGFP+ OMPs and osteoblasts (upper panel) maintain their lineage identity after fin regeneration (lower panel). n = 8. Scale bar, 25 μm. (c) Sections of a fin ray containing EGFP+ CMPs before and after regeneration. Only CMPs were generated after regeneration (arrowheads). n = 6. Scale bar, 25 μm. (d) Schematic of the experimental procedure for OPC ablation. The Tg was treated with metronidazole (Mtz) for 4 days to ablate joint OPCs and allowed to regenerate for 19 days (total 23 days). (e) Fluorescence images showing OPC replenishment from OMPs (red fluorescence). Replenished OPCs in joints look yellow. n = 6. Scale bar, 50 μm. (f) Fluorescence images of replenished OPCs in the fin ray with a CMP lineage label (red fluorescence). Replenished OPCs do not show red fluorescence. n = 4. Scale bar, 50 μm.