Fig. 4

Fig. 4 Infrared-mediated image restoration provides high-contrast deep-tissue time-lapse imaging of living biological systems.

a, 3D reconstruction of live Tg(h2b:gfp) zebrafish larva images. Orange boxes represent the regions shown in b,c. Scale bar, 100 µm. b,c, Individual z planes at detection depth of 100 µm and 250 µm, respectively for the fish larva shown in a. Endogenous GFP (top), IR² reconstructed images (bottom). Scale bar, 100 µm. d, Kymograph representing the information content gain relative to the GFP images, for all the images in the time-lapse dataset and as a function of detection depth. Line plots to the right and the bottom represent the depth- and time-average information content gain. e, 3D reconstruction of live (Tg(His2AV-GFP)) fly larva images. Green opaque planes represent the sample sections shown in f. Scale bar, 100 µm. f, Individual z plane for the fly images shown in e. Endogenous GFP (top), IR² reconstructed images (bottom). Red-highlighted time points are shown in g,h. Scale bar, 100 µm. g,h, Spatial mapping of the information content gain for the two individual z planes shown. Scale bar, 100 µm. i, Kymograph of the information content gain as a function of time and detection depth. Line plots represent the depth- and time-average information content gain.