ZFIN Image: Raas et al., 2024, Figure 4

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Figure Caption

Figure 4

Zebrafish peroxisomal reporter construct and characterization of effects on peroxisomes. (A) Schematic of the mCherry-SKL construct and transient expression in zebrafish. Confocal z-stack, lateral view of the trunk, live 3 dpf larva, green channel, DCFH-DA (dacetyldichlorofluorescein). (B) Live confocal images and paired IMARIS (3D reconstruction) image (below), of mCherry-SKL (red) containing peroxisomes in the hair cells (blue) of larval zebrafish neuromast at 6 dpf in zebrafish overexpressing WT or N237S ACOX1. (C) Size and distance to nearest neighbors in zebrafish overexpressing WT or N237S ACOX1, showing a decreased density of peroxisomes in these cells. (D) qRT-PCR for expression of genes that affect the abundance of peroxisome: pparab, pex11a, pex11b, pex11g, and nrb1b.

Acknowledgments

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