Figure 4

Figure 4

Lithium does not increase the expression and activity of non-phosphorylated β-catenin. WT (circles) and 2b4^he2b5^ho mutant (VWM; squares) mice (n = 8 per treatment, per genotype) were i.p. injected daily with placebo (black) or 200 mg/kg lithium (blue) for 1 week. Mice were terminated approximately 8 h after the final injection. Cerebellar RNA and protein samples were derived from the same lysate. Levels of non-phosphorylated β-catenin were determined using the Western blot (n = 3–4). Lithium treatment effects on indicated β-catenin-regulated markers were determined with qPCR (n = 6–8) (Sorcs1 and Nrxn as reference). Lithium differences were analyzed with two-way ANOVAs (Supplementary Data Sheet S1). The graphs show individual and mean values, +/− SEM. Raw data are available in Supplementary Data Sheet S2.