Fig 1

Fig 1

Rac2-deficient zebrafish larvae are susceptible to A. fumigatus infection. (A) rac2^+/− adult fish were crossed to each other, and the resulting larvae were injected with TBK1.1 (Af293) spores. Survival was monitored for 7 dpi, and larvae were genotyped at the conclusion of the experiment. Average injection CFUs = 46. Data represent three pooled replicates. P values and hazard ratios were calculated by Cox proportional hazard analysis. (B) Larvae previously treated with rac2 or standard control MO were injected with TBK1.1 (Af293) spores. At 0–5 dpi, larvae were homogenized and plated to evaluate CFUs. CFU counts were normalized to the average injection dose for each group for each replicate. CFUs were evaluated from four to eight larvae per group per day per replicate. Average injection CFUs: control = 46, rac2 = 56. Plotted data represent emmeans ± SEM from four pooled experiments; P values were calculated by analysis of variance (ANOVA).