ZFIN Image: Kahsay et al., 2024, Figure 2.

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Figure Caption

Figure 2.

Obscurin distribution in zebrafish EOMs. (A–C) Longitudinal sections of 10-month-old wild-type zebrafish EOMs immunolabeled with antibodies against obscurin (green in A), and myomesin (red in B; merged image in C). Obscurin is localized in the M-band identified by staining with antibodies against myomesin (red in B). (D–F) Immunolabeling against obscurin (green in D) and F-actin (red in E; merged in F) showed the presence of obscurin in the vast majority of the myofibers in zebrafish EOMs. Lack of obscurin was also noted in a small subgroup of myofibers (arrowheads). Longitudinal sections of zebrafish EOMs immunolabeled with obscurin (green in G) at NMJs identified by α-bungarotoxin (red in H; merged in I) showed that obscurin was not enriched at NMJs as in other muscles. Immunolabeling of obscurin (arrowheads, green in J, M) at MTJs of zebrafish EOMs labeled by laminin (red in K, N; merged in L and O).

Acknowledgments

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