Figure 4

Figure 4

Upregulated expression of amh and gsdf was observed in cyp19a1a−/− fish of PGC-rich and PGC-less groups at 23 dpf. (A) A schematic showing the procedure of the classification according to PGC area and gene expression analyses. (B) The bi-potential gonad area of the selected cyp19a1a+/+ and cyp19a1a−/− fish at 23 dpf. Dark green circles: top three bipotential gonad area. Brown circles: last three bi-potential gonad area. (C,D) Representative images of the selected cyp19a1a+/+ and cyp19a1a−/− fish from the PGC-rich group at 23 dpf. EGFP-labeled gonads were pointed by white arrows. Scale bar, 300 µm. (E,F) Representative images of the selected cyp19a1a+/+ and cyp19a1a−/− fish from the PGC-less group at 23 dpf. EGFP-labeled gonads were pointed by white arrows. Scale bar, 300 µm. (G,H) Relative expression of amh and gsdf in cyp19a1a+/+ and cyp19a1a−/− fish of the PGC-rich and PGC-less groups at 23 dpf. n.s., no significance. * p < 0.05. Different letters in the bar charts represent significant differences.