Figure 6

Figure 6

Transit amplifying cells express GluN1. (A–F) Immunohistochemistry against GluN1 and PCNA in 3 dpf wild-type fish. Panels on the right are magnified versions of those on the left. Asterisks indicate example regions of neuropil in (A, C, & E). (A & B) GluN1 channel showing robust plasmelemmal and neuropilar dendritic expression of GluN1. Arrows indicate example cells in abventicular and cell-rich parenchymal locations (A) and illustrate plasmelemmal GluN1 expression (B). (C & D) PCNA channel showing ventricular PCNA⁺ NSCs and abventricular and cell-rich parenchymal PCNA⁺ tACs cells. Arrows indicate the same cells as in (A & B) and illustrate that the same cells express both PCNA and GluN1. (E & F) Merged GluN1 and PCNA channels. Arrows indicate same cells as in (A, B, C, & D) arrow heads indicate example PCNA⁺ tAC with GluN1 puncta.