Figure 4

Figure 4

Radial glia are not increased in the forebrain of grin1^−/− fish. (A–F) GFAP expression (index of RGCs) of 3 dpf wild-type (A, C, & E) and grin1^−/− (B, D, & F) fish at three gross anatomical levels of the forebrain (see "Materials and methods"). Brain schematics above images indicate the gross anatomical regions sampled. Brain schematics after Mueller & Wullimann (2016). (G–I) Box and whisker plots of CFI analysis. Wild type and grin1^−/− sample sizes and p value (t-test): Precommissural telencephalon (G), 7, 7; p = 0.38. Postcommissural telencephalon (H), 7, 7; p = 0.17. Diencephalon (I), 6, 7; p = 0.53. Significance (t-test) is indicated (ns, not significant).