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Fig. 2 (A, B) Morphological features observed at the light microscope and quantitative analysis of the phenotypes of 24 hpf embryos injected with human wtMYO or mutMYO mRNA. (C) Representative lateral views of a single confocal projection (z-stack) of mutMYO, wtMYO, and control embryos at 48 hpf stained with phalloidin. Skeletal muscle fibers appear less consistent and compact in mutMYO-injected embryos compared to wtMYO-injected or not injected (control) larvae. (D) Transmission electron micrographs of skeletal muscles from embryos injected with wtMYO mRNA (a, b) and mutMYO mRNA (c, d). Note the irregular arrangement and the heterogeneous thickness of myofibres in mutant embryos (c) compared to controls (a). High magnification images (b, d) show in mutant embryos well-organized Z lines but I-bands wider than in controls. Bars: 1 μm (a, c); 200 nm (b, d). [Colour figure can be viewed at wileyonlinelibrary.com]