Fig. S5

Fig. S5 Image processing procedures for cryo-EM of E. coli 70S ribosomes, related to Figure 6 Image processing of E. coli 70S ribosomes complexed with tRNAiMet at P-site Q-tRNAs at the A-site. (A–F) (A) Human tRNAAsp bearing G34 and the GAU codon at the A-site (PDB: 7Y7C , EMDB: EMD-33660), (B) human tRNAAsp bearing Q34 and the GAU codon at the A-site (PDB: 7Y7D, EMDB: EMD-33661), (C) human tRNAAsp bearing manQ34 and the GAU codon at the A-site (PDB: 7Y7E, EMDB: EMD-33662), (D) human tRNAAsp bearing manQ34 and the GAC codon at the A-site (PDB: 7Y7F, EMDB: EMD-33663), (E) human tRNATyr bearing galQ34 and the UAU codon at the A-site (PDB: 7Y7G, EMDB: EMD-33664), (F) human tRNATyr bearing galQ34 and the UAC codon at the A-site (PDB: 7Y7H, EMDB : EMD-33665). The subgroup others includes A-site vacant 70S and low-resolution particles. (G and H) Gold standard FSC curves (G) and refined model versus map FSC curves (H) of cryo-EM models. Black, tRNAAsp (manQ34) with the GAU codon (PDB: 7Y7E , EMDB: EMD-33662), blue, tRNAAsp (manQ34) with the GAC codon (PDB: 7Y7F, EMDB: EMD-33663), red, tRNATyr (galQ34) with the UAU codon (PDB: 7Y7G, EMDB: EMD-33664), green, tRNATyr (galQ34) with the UAC codon (PDB: 7Y7H, EMDB: EMD-33665), orange, tRNAAsp (G34) with the GAU codon (PDB: 7Y7C, EMDB: EMD-33660), purple, tRNAAsp (Q34) with the GAU codon (PDB: 7Y7D, EMDB : EMD-33661). (I) Color-coded local resolution distribution of cryo-EM map for each ribosome complex as indicated. Blue circles indicate the A-sites.