Fig. S4

Fig. S4 Image processing procedures for cryo-EM of human 80S ribosomes, related to Figure 6 Image processing of human 80S ribosomes complexed with Q-tRNAs at both A- and P-sites. (A–C) (A) Human tRNAAsp bearing Q34 and the GAU codon at the A- and P-sites (PDB: 8JDJ , EMDB: EMD-36178), (B) Human tRNAAsp bearing manQ34 and the GAU codon at the A- and P-sites (PDB: 8JDK, EMDB: EMD-36179), (C) human tRNATyr bearing galQ34 and the UAU codon at the P-site in the classical state (PDB: 8JDL, EMDB: EMD-36180), and at the A- and P-sites in the hybrid state (PDB: 8JDM, EMDB : EMD-36181). The subgroup “others” includes A-site vacant 80S and low-resolution particles. (D and E) Gold standard Fourier shell correlation (FSC) curves (D) and refined model versus map FSC curves (E) of cryo-EM models. Black, tRNAAsp (Q34) with the GAU codon (PDB: 8JDJ , EMDB: EMD-36178), blue, tRNAAsp (manQ34) with the GAU codon (PDB: 8JDK, EMDB: EMD-36179), Red, tRNATyr (galQ34) with the UAU codon in the classical state (PDB: 8JDL, EMDB: EMD-36180), Green, tRNATyr (galQ34) with the UAU codon in the hybrid state (PDB: 8JDM, EMDB : EMD-36181). (F) Color-coded local resolution distribution of cryo-EM maps of human 80S ribosome complexed with tRNAAsp bearing Q34 (left) and manQ34 (right). Red circles indicate the A-sites. (G) Color-coded local resolution distribution of cryo-EM maps of human 80S ribosome complexed with tRNATyr bearing galQ34 in the hybrid state (left and middle) and in the classical state (right). Red circles indicate the P- and A-sites.