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Fig. 6 Dimercaprol increases GSH and attenuates acute ‘seizure-like’ swim behavior in wildtype larvae. 5-7 dpf wildtype (WT) zebrafish larvae were treated with 1, 10, 30 or 100 μM dimercaprol for 4 h. GSH levels were measured by HPLC. (A) Larval GSH levels. WT larvae were pre-treated with 100 μM DMP for 4 h and stimulated with 10 mM PTZ for 40min. Larval locomotion/swim behavior was measured by the Noldus DanioVision system running the EthoVisionXT software. (B) Change in larval swim velocity correlated to ‘seizure-like’ behavior. Data are represented as mean ± SEM (error bars). ***p < 0.001, ****p < 0.0001, ns: not significant versus vehicle control; βp<0.05 versus 10 mM PTZ by one-way ANOVA with Dunnett's post-hoc test (A) and Tukey's post-hoc test (B). n = 50–70 larvae pooled/grp (HPLC assay) and n = 10 larvae/grp (Noldus assay). N = 4–5 experimental replicates. Mean ± SD of raw data values of GSH normalized to total protein (nmol/mg protein) are stated in parentheses (GSH) for each treatment group: a) Veh ctrl (14.9 ± 7.7); b) 1 μM DMP (14.9 ± 5.9); c) 10 μM DMP (16.2 ± 6.9); d) 30 μM DMP (15.5 ± 4.7); and e) 100 μM DMP (21.7 ± 5.4) (6A).