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Fig. 5 GSH depletion oxidizes TSC1, TSC2, and TBC1D7 in the mTORC1 pathway. Primary neuronal-glial cerebrocortical cultures were treated with 300 μM BSO for 24 h and the total proteins TSC1, TSC2, and TBC1D7 normalized to loading control β-tubulin were probed for by redox-western blotting. 30 nM PAO was used as positive control. (A) Representative blot, and (B) Blot quantification for TSC1. (C) Representative blot, and (D) Blot quantification for TSC2. (E) Representative blot, and (F) Blot quantification for TBC1D7. Data are represented as mean ± SEM (error bars). *p < 0.05, **p < 0.01 versus vehicle control by one-way ANOVA with Dunnett's post-hoc test. n = 3/grp. N = 3 experimental replicates.