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Fig. 2 4AP-induced hyperexcitability aberrantly activates mTORC1. Primary neuronal-glial cerebrocortical cultures were pretreated with 30 μM dimercaprol for 4 h and then stimulated with 1 mM 4AP for an additional 4 h. mTORC1 activity represented by phospho-S6 (p-S6)/total S6 (t-S6) was assessed by immunoblotting. 20 nM rapamycin was used as procedural/positive control. (A) Representative blot, and (B) Blot quantification. Data are represented as mean ± SEM (error bars). ****p < 0.0001, ns: not significant versus vehicle control, ββp<0.01 versus 1 mM 4AP by one-way ANOVA with Tukey's post-hoc test. n = 3/grp. N = 4 experimental replicates.