Fig. 7

Fig. 7 Cebp1 is the orthologue of human C/EBPε^P27 in suppressing eosinophilopoiesis.

A Full Cebpe knockout affects both neutrophils and eosinophils in mice. BM cells of Cebpe^+/+ and Cebpe^−/− mice were collected, stained with 15 different antibodies, and applied to flow cytometry analysis. The UMAPs were shown, representing the clustering information of mouse BM cells. Red circles marked the eosinophils, while the black-dotted regions marked the atypical granulocytes. The entire annotation information was shown on the right panel. B Neutrophil-specific Cebpe knockout induces atypical granulocytes in mice. BM cells of Cebpe^fl/fl and Mrp8^creCebpe^fl/fl mice were collected, stained with 15 different antibodies, and applied to flow cytometry analysis. The UMAPs were shown, representing the clustering information of mouse BM cells. C Overexpression of human C/EBPε^p27 mimicked Cebp1 phenotype. WT embryos were injected with coro1a-drived overexpression plasmids at 1-cell stage, then screened at 1 dpf based on eGFP signals, and finally collected at 7 dpf for WISH. Eosinophil (red arrowhead) numbers were decreased in larvae injected with coro1a:cebp1-eGFP (DrCebp1) or coro1a:CEBPE^p27-eGFP (HsC/EBPε^p27) plasmid, while remained unchanged in other groups (bar = 200 μm). Quantification was performed using Student’s t test (each group was only compared with coro1a:eGFP (eGFP) control, two-sided, mean ± SEM, ns represents no significance). Sample size: eGFP, n = 17; DrCebp1, n = 14; HsC/EBPε^p27, n = 15; HsC/EBPε^p14, n = 20; HsC/EBPε^p30, n = 22; HsC/EBPε^p32, n = 23; MmC/EBPε, n = 18. Three independent experiments were conducted. Source data are provided as a Source Data file.