Fig. 5

Fig. 5 Cebpβ promotes the commitment of the eosinophil lineage.

A Cebpβ is a positive regulator of eosinophilopoiesis. Eosinophil (red arrowhead) numbers were found to decrease in 7 dpf cebpb mutant larvae (bar = 200 μm). Quantification was performed using Student’s t-test (two-sided, mean ± SEM). Sample size: Sibling, n = 51; cebpb^−/−, n = 13. Three independent experiments were conducted. B Overexpression of cebpb enhanced eosinophilopoiesis. Eosinophil (red arrowhead) numbers were increased in 7 dpf Tg(hsp70:cebpb-eGFP) larvae (bar = 200 μm). Quantification was performed using Student’s t test (two-sided, mean ± SEM). Sample size: Control, n = 23; hsp70:cebpb-eGFP, n = 10. Three independent experiments were conducted. C UMAP showing the scRNA-Seq clustering information. KM cells of WT and cebpb^−/− zebrafish were integrated and analyzed by scRNA-Seq analysis. The dotted line-surrounding regions indicate the eosinophil lineage of each sample, which were further subclustered and the enlarged view is shown. D Eosinophil lineage profile with cebpb mutation. Percentages of eosinophil lineage cells of different stages were analyzed in the KM of WT and cebpb^−/− zebrafish (n = 3, Student’s t test, two-sided, mean ± SEM, ND means not detectable). Source data are provided as a Source Data file.