Fig. 3

Fig. 3 Cebp1 inhibits the commitment of eosinophils.

A Mutation of cebp1 enhanced eosinophilopoiesis. Eosinophil (red arrowhead) numbers were increased in 7 dpf cebp1 mutant larvae (bar = 200 μm). Quantification was performed using Student’s t-test (two-sided, mean ± SEM). Sample size: Sibling, n = 27; cebp1^−/−, n = 10. Three independent experiments were conducted. B Overexpression of cebp1 inhibited eosinophilopoiesis. Eosinophil (red arrowhead) numbers were decreased in 7 dpf Tg(hsp70:cebp1-eGFP) larvae (bar = 200 μm). Quantification was performed using Student’s t test (two-sided, mean ± SEM). Sample size: Control, n = 23; hsp70:cebp1-eGFP, n = 19. Three independent experiments were conducted. C UMAP showing the scRNA-Seq clustering information. KM cells of WT and cebp1^−/− zebrafish were integrated and analyzed by scRNA-Seq analysis. The purple, green, and yellow dotted line-surrounding regions respectively indicate the eosinophil lineage, neutrophil lineage, and atypical cell cluster of each sample. Eosinophil lineage cells of each sample were further subclustered and the enlarged view (black-boxed region) is shown. D Subcluster annotation reference. The expression patterns of eslec and the markers of each cluster (Eb-P: ncf2 and myca; EoP: mcm2 and pcna; pre.Eos: agr2 and pdia2; mat.Eos: fcer1gl and cxcr4b) are shown by violin plots. E Eosinophil lineage profile with cebp1 mutation. Percentages of eosinophil lineage cells of different stages were analyzed in WT and cebp1^−/− zebrafish (n = 3, Student’s t test, two-sided, mean ± SEM). Source data are provided as a Source Data file.